PKC β Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9941M-50, BS9941M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P05771

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:2000
IHC/ICC/IF: 1:50-1:200
FC: 1:50-1:100

Background: PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+. On the other hand, nPKC δ and ε, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of PKC β and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 82 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PKC β Rabbit mAb at 1:1000 dilution
Lane 1: Raji whole cell lysate, Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC β antibody.Counter stained with hematoxylin., ICC staining PKC beta 2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Protein kinase C beta type; PKC-B; PKC-beta; PKCB; PRKCB1; PRKCB

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified