Recombinant Human COL8A1 Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH032267-10, PKSH032267-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: COL8A1

Target Synonym: Collagen Alpha-1(VIII) Chain; Endothelial Collagen; Vastatin; COL8A1; C3orf7

UNIProt ID: P27658

Research Areas: Signal Transduction; Cardiovascular;

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-6His

Accession: P27658

Background: Collagen alpha-1(VIII) chain, also known as endothelial collagen, C3orf7 and COL8A1, can be cleaved into vastatin chain. COL8A1 is a short chain collagen and a major component of the basement membrane of the corneal endothelium. COL8A1 forms homotrimers, or heterotrimers in association with alpha 2(VIII) type collagens. Four homotrimers can form a tetrhedron stabilized by central interacting C-terminal NC1 trimers. COL8A1 contains one C1q domain and is primarily expressed in the subendothelium of large blood vessels. The expression level can be up-regulated during vascular injury, in atherosclerosis and in diabetes. COL8A1 may have a role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.

Sequence: Gly28-Met744

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 71.6 kDa

Observed MW: 58-85 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only