mAChR M4 (P277) Polyclonal Antibody

Catalogue Numbers: BS3236-50, BS3236-100

Sizes: 50µl, 100µl

Alternative Name: Muscarinic acetylcholine receptor M4,CHRM4

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08173

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The muscarinic acetylcholine receptors (mAChR) mediate a variety of cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels. The mAChRs transducer signals by coupling to G-proteins, which then modulate several downstream effector proteins and ion channels. Five mAChR subtypes have been identified, designated M1 to M5. The five receptor subtypes show distinct patterns of tissue distribution, as well as distinct pharmacological and functional properties.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: mAChR M4 (P277) polyclonal antibody detects endogenous levels of mAChR M4 protein

Bioworld Molecular Weight: ~ 53 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of mAChR M4 (P277) pAb at 1:500 dilution
Lane 1: H1792 whole cell lysate(40ug)
Lane 2: MCF-7 whole cell lysate(40ug)
Lane 3: K562 whole cell lysate(40ug)
Lane 4: C6 whole cell lysate(40ug)
Lane 5: SP2/0 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 241-290 of Human mAChR M4.

Conjugate: Unconjugated

Modification: Unmodified