EBP50 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90442-50, BS90442-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14745(Human) Q9JJ19(Rat)

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC

All Applications: WB:1:1,000-1:2,000
IHC:1:50-1:200

Background: The Na+/H+ exchange protein (NHE3) functions in transepithelial Na+ absorption and is primarily expressed in the intestinal and renal brush border membrane. NHE3 regulatory factor 1 (NHERF-1) interacts with NHE3 through two PDZ (for PSD-95, Discs-large and ZO-1 homology) domains, which are protein-protein interaction modules that associate with specific carboxy-terminal motifs on target proteins. Also known as EBP50, NHERF-1 facilitates cAMP inhibition of NHE3 to decrease Na+ adsorption. NHERF-1 functions as a scaffold for an essential multiprotein complex of Ezrin and NHE3 for cAMP-mediated phosphorylation and consequent inhibition of NHE3. The amino-terminal PDZ domain regulates the dimerization of NHERF-1 in vivo. G protein-coupled receptor kinase 6A phosphorylates NHERF-1 at Ser 289 via a PDZ domain-mediated interaction. NHERF-2, also known as E3KARP, is a ubiquitously expressed protein which also functions in NHE2 regulation.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: EBP50 polyclonal antibody detects endogenous levels of EBP50 protein.

Bioworld Molecular Weight: 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of EBP50 on MCF-7 cells lysates using anti-EBP50 antibody at 1/1,000 dilution., Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-EBP50 antibody. Counter stained with hematoxylin.

Alternative Name: NHERF-1, EBP50, SLC9A3R1, EBP 50, EBP50, Ezrin radixin moesin binding phosphoprotein 50, Ezrin-radixin-moesin-binding phosphoprotein 50, Na(+)/H(+) exchange regulatory cofactor NHE RF, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Na+/H+ exchange regulatory co factor, NHERF 1, NHERF, NHERF-1, NHERF1, NHRF1, NPHLOP2, Regulatory cofactor of Na(+)/H(+) exchanger, Slc9a3r1, Sodium hydrogen exchanger regulatory factor 1, Sodium-hydrogen exchanger regulatory factor 1, Sodium/hydrogen exchanger regulatory factor 1, Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1, Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1, Solute carrier family 9 isoform 3 regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1, Solute carrier family 9 member 3 regulator 1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified