EKLF (W318) Polyclonal Antibody

Catalogue Numbers: BS2303-50, BS2303-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13351

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IHC IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: The Kruppel-type zinc finger transcription factors comprise a conserved family of DNA binding proteins that are important in developmental regulation. The Kruppel zinc finger transcription factor was initially identified in Drosophila as a segmentation gene. Kruppel-like factors that have been characterized in mammals include EKLF, LKLF and GKLF. EKLF is expressed principally in erythroid tissues, and LKLF expression is limited to the lung. GKLF is found predominantly in gut and has been shown to be expressed during growth arrest.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EKLF (W318) polyclonal antibody detects endogenous levels of EKLF protein. This antibody also recognizes lKLF (51 kDa), GKLF (56 kDa), and UKLF (33 kDa).

Extra Notes: Western blot (WB) analysis of EKLF (W318) pAb at 1:500 dilution
Lane1:C6 whole cell lysate(40ug)
Lane2:SP2/0 whole cell lysate(40ug)
Lane3:MCF-7 whole cell lysate(40ug)
Lane4:K562 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Krueppel-like factor 1; Erythroid krueppel-like transcription factor; EKLF; KLF1; EKLF

Immunogen: Synthetic peptide, corresponding to amino acids 288-335 of Human EKLF.

Conjugate: Unconjugated

Modification: Unmodified