Recombinant Cynomolgus 4-1BB (C-Fc)

Size: 50ug

Catalogue Number: EPT062-50

Citations, Manuals and MSDS Available upon request.

Background: Tumor necrosis factor receptor superfamily member 9 (TNFRSF9), also known as CD137 and 4-1BB, is an inducible T cell surface protein belonging to the tumor necrosis factor receptor superfamily. It is a single-pass type I membrane protein which contains 4 TNFR-Cys repeats. The human and mouse proteins share 60% amino acid sequence identity. CD137 is expressed by mesenchymal cells, including endothelial cells, chondrocytes, and cells of the central nervous system. CD137 is also broadly expressed by cells of the human immune system, is broadly expressed by cells of the human immune system, including activated CD8+ and CD4+ T cells, activated natural killer (NK) cells, follicular dendritic cells (FDCs) and monocytes. CD137 has diverse roles in the immune response, the one key function is to promote the survival of both T cells and dendritic cells by binding the cognate ligand CD137L (4-1BBL).

Alternate Names: CD137; ILA; TNFRSF9; 4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA

Description: Recombinant Cynomolgus 4-1BB ligand Receptor is produced by our Mammalian expression system and the target gene encoding Leu24-Gln186 is expressed with a Fc tag at the N-terminus.

Accession: A9YYE7

Molecular Weight: 44.4 KDa

Apparent Molecular Weight: 60 KDa, reducing conditions

Storage Condition: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Biological Activity: Immobilized Cynomolgus 4-1BB-Fc (Cat#CB19) at 2μg/ml (100 μl/well)can bind Human 4-1BBL-His-Flag (Cat#C17A). The ED50 of Human 4-1BBL-His-Flag (Cat#C17A)is 0.418 ug/ml.

Redissolve: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

Delivery Condition: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Research Use Only