APAF1 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-65705-60, E-AB-65705-120, E-AB-65705-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: APAF1

Target Synonym: APAF1; APAF-1; CED4

Research Areas: Cancer, Cell Biology, Metabolism

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human

Application: WB

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: O14727

Background: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human APAF1 (NP_863651.1).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000

Calculated MW: 37kDa/133kDa/135kDa/137kDa/140kDa/141kDa

Observed MW: 142kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only