Recombinant Human MECR Protein (His Tag)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSH033131-10, PKSH033131-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: MECR

Target Synonym: Trans-2-Enoyl-CoA Reductase Mitochondrial; Nuclear Receptor-Binding Factor 1; HsNrbf-1NRBF-1; MECR; NBRF1

UNIProt ID: Q9BV79

Research Areas: Signal Transduction; metabolism;

Target Species: Human

Expression Host: HEK293 Cells

Fusion Tag: C-6His

Accession: Q9BV79

Background: Trans-2-Enoyl-CoA Reductase Mitochondrial (MECR) belongs to the zinc-containing alcohol dehydrogenase family. MECR localizes to the mitochondrion. It is highly expressed in skeletal and heart muscle and expressed at lower levels in the placenta, liver, kidney and pancreas, with weakly or no expression in the lung. MECR exists as a homodimer, which catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH-dependent manner with preference to medium chain length substrate. MECR may take part in the mitochondrial synthesis of fatty acids.

Sequence: Pro54-Met373

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 35.7 kDa

Observed MW: 39 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only