C9 (H216) Polyclonal Antibody

Catalogue Numbers: BS3841-50, BS3841-100

Sizes: 50µl, 100µl

Alternative Name: Complement component C9; Complement component C9a; C9

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P02748

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: C9 is synthesised in the liver and monocytes, and is a plasma protein consisting of a single polypeptide chain of molecular weight 71kDa. Normal plasma concentration is 60mg/L. C9 forms part of the membrane attack complex (MAC) the cytolytic terminal complex of the complement pathways. C9 binds to the membrane associated C5b-8, binding of C9 to C5b-8 leads to the circular polymerisation of 12-18 C9 molecules. This is the basis of the hydrophilic, protein-walled, trans-membrane channel formed by the MAC, which leads to cell lysis and destruction.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: C9 (H216) polyclonal antibody detects endogenous levels of Complement component C9 protein.

Bioworld Molecular Weight: ~ 63 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of C9 (H216) polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 171-220 of Human C9.

Conjugate: Unconjugated

Modification: Unmodified