GAP43 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90559-50, BS90559-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06837

Host: Rabbit

Reactivity: Human, Mouse, rat

Applications: WB, ICC IHC FC

All Applications: WB:1:1,000
ICC:1:200
IHC:1:200
FC:1:100-1:200

Background: GAP43, is a nervous tissue-specific cytoplasmic protein that can be attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4. This sequence targets GAP43 to lipid rafts. It is a major protein kinase C (PKC) substrate and is considered to play a key role in neurite formation, regeneration, and plasticity. The role of GAP-43 in CNS development is not limited to effects on axons: It is also a component of the centrosome, and differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. As a consequence, in the cerebellum, the neuronal precursor pool fails to expand normally and the cerebellum is significantly smaller.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: GAP43 polyclonal antibody detects endogenous levels of GAP43 protein.

Bioworld Molecular Weight: 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of GAP43 on different cell lysates using anti-GAP43 antibody at 1/1000 dilution.
Positive control:
Lane 1: Rat brain
Lane 2: Mouse brain
Lane 3: Mouse heart
Lane 4: Human skeletal muscle
Lane 5: N2A
Lane 6: A172
Lane 7: Human heart, ICC staining GAP43 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: NEUM, Gap43, Axonal membrane protein GAP 43, Axonal membrane protein GAP-43, B 50, Calmodulin binding protein P 57, F1, GAP 43, GAP43, Growth Associated Protein 43, Growth-associated protein 43, Nerve Growth Related Peptide, Nerve growth related peptide GAP43, NEUM, Neural phosphoprotein B 50, Neural phosphoprotein B-50, Neuromodulin, Neuron growth associated protein 43, PP46, Protein F1, QtrA-11580, QtrA-13071,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified