Cytokeratin 15 Monoclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: MB65999-50, MB65999-100 Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Swiss-Prot: P19012 Host: Mouse Reactivity: Human Applications: WB, IHC All Applications: WB (1/500 - 1/1000), IHC (1/100 - 1/300) Background: Cytokeratin 15 (CK15, K15, K1CO, keratin15, type I cytoskeletal 15) is an intermediate filament (IF) type I protein that is responsible for the mechanical integrity of epithelial cells. Keratin family members are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains, and are clustered in a region on chromosome 17q21.2. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceus. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins. Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Recognizes endogenous levels of Cytokeratin 15 protein. Bioworld Molecular Weight: ~ 49 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Immunohistochemical analysis of Cytokeratin 15 staining in human cervical squamous carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of Cytokeratin 15 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of Cytokeratin 15 staining in human squamous cell lung carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Alternative Name: KRTB; Keratin type I cytoskeletal 15; Cytokeratin-15; CK-15; Keratin-15; K15 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Cytokeratin 15. The exact sequence is proprietary. Conjugate: Unconjugated Modification: Unmodified