CD6 Recombinant Protein

Catalogue Number: NCP0218-500, NCP0218-1000

Sizes: 500ug, 1mg

Swiss-Prot: P30203

Host: E.coli

Tag: His-tag

AA Sequence: CATCCGAGTCCGGCACCGCCTGATCAGCTGAATACCAGCAGCGCAGAAAGTGAACTGTGGGAACCGGGTGAACGTCTGCCGGTGCGCCTGACCAATGGCAGCAGCAGTTGCAGCGGCACCGTGGAAGTGCGCCTGGAAGCAAGTTGGGAACCGGCATGCGGTGCCCTGTGGGATAGCCGTGCAGCCGAAGCCGTGTGCCGCGCACTGGGTTGTGGCGGTGCAGAAGCCGCCAGCCAGCTGGCACCGCCTACCCCTGAACTGCCGCCGCCTCCTGCCGCTGGTAATACCAGCGTTGCAGCAAATGCAACCCTGGCAGGCGCACCGGCACTGCTGTGCAGTGGCGCAGAATGGCGTCTGTGCGAAGTTGTTGAACATGCATGTCGCAGTGATGGTCGCCGTGCCCGCGTTACCTGTGCCGAAAATCGTGCCCTGCGTCTGGTGGATGGCGGTGGCGCATGCGCAGGTCGTGTTGAAATGCTGGAACATGGCGAATGGGGCAGTGTGTGTGATGATACCTGGGATCTGGAAGATGCACATGTTGTGTGTCGCCAGCTGGGCTGTGGCTGGGCCGTGCAAGCCCTGCCTGGTCTGCACTTCACCCCGGGTCGTGGCCCGATTCATCGCGATCAGGTTAATTGCAGCGGTGCAGAAGCATATCTGTGGGATTGTCCGGGTCTGCCGGGCCAGCATTATTGTGGCCATAAAGAAGATGCAGGTGCCGTGTGCAGTGAACATCAGAGCTGGCGTCTGACCGGTGGTGCCGATCGCTGCGAAGGCCAGGTTGAAGTTCACTTCCGCGGCGTGTGGAATACCGTGTGCGATAGCGAATGGTATCCGAGCGAAGCCAAAGTGCTGTGCCAGAGTCTGGGTTGCGGTACCGCCGTGGAACGTCCGAAAGGTCTGCCGCATAGTCTGAGTGGTCGCATGTATTATAGCTGTAATGGTGAAGAACTGACCCTGAGCAATTGTAGCTGGCGCTTCAATAATAGTAATCTGTGCAGCCAGAGTCTGGCCGCACGCGTTCTGTGCAGCGCCAGTCGCAGCCTGCATAATCTGAGTACCCCGGAAGTTCCGGCCAGTGTTCAGACCGTGACCATTGAAAGTAGTGTGACCGTTAAAATTGAAAATAAGGAAAGCCGCGAACTGATGCTG

Restriction Sites: NdeI-XhoI

Background: T cell differentiation antigen CD6 is a cell adhesion molecule expressed on immature thymocytes and mature T cells, and has also been detected on a subset of B cells and NK cells within the immune system. CD6 mediates cell-cell interactions through it’s binding partner CD166/ALCAM, and contributes to the formation and maturation of the immunological synapse. CD6 functions as a co-stimulatory receptor, promoting T cell activation and proliferation through the TCR/CD3 complex signaling cascade. Studies have shown CD6 can be glycosylated, hyperphosphorylated on serine and threonine residues, and phosphorylated on tyrosine residues, each of which can differentially effect the function and signaling of this molecule. CD6 also functions as a calcium-dependent pattern receptor that binds and aggregates Gram-positive and Gram-negative bacteria. In response to lipopolysaccharide, CD6 mediates activation of the inflammatory response and secretion of pro-inflammatory cytokines.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~44kDa

Notes: For research use only, not for use in diagnostic procedure.