AGPAT1 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS6317-50, BS6317-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99943

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:100

Background: This gene encodes an enzyme that converts lysophosphatidic acid (LPA) into phosphatidic acid (PA). LPA and PA are two phospholipids involved in signal transduction and in lipid biosynthesis in cells. This enzyme localizes to the endoplasmic reticulum. This gene is located in the class III region of the human major histocompatibility complex. Alternative splicing results in two transcript variants encoding the same protein.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 32kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using AGPAT1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug AGPAT1 antibody. Western blot was performed from the immunoprecipitate using AGPAT1 antibody at a dilution of 1:1000.

Alternative Name: AGPAT1; 1-AGPAT1; G15; LPAAT-alpha; LPAATA

Immunogen: Recombinant fusion protein of human AGPAT1(NP_006402.1).

Conjugate: Unconjugated

Modification: Unmodified