Rab 6A (K144) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3920-50, BS3920-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P20340

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:10~1:100

Background: Rab proteins are also an integral part of endocytic pathways. Rab 6A is a ubiquitously expressed member of the Rab family of proteins and localizes to the Golgi membrane where it regulates retrograde transport from the late endosomes via the Golgi to endoplasmic reticulum (ER) pathway. Three isoforms exist due to alternative splicing events, namely the ubiquitously expressed isoforms Rab 6A' and Rab 6A, and the brain-specific isoform Rab 6B.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Rab 6A (K144) polyclonal antibody detects endogenous levels of Rab 6A protein.

Bioworld Molecular Weight: ~ 24 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Rab 6A (K144) pAb at 1:500 dilution
Lane 1: MCF-7 whole cell lysate (40ug)
Lane 2: HepG2 whole cell lysate (40ug)
Lane 3: K562 whole cell lysate (40ug)
Lane 4: The Testis tissue lysate of Mouse (40ug)
Lane 5: The Testis tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of Rab 6A (K144) pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasmic and membranous staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of Rab 6A (K144) pAb in paraffin-embedded human liver carcinoma tissue at 1:50, showing cytoplasmic and membranous staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunoprecipitation of HEK293T cell lysate using Rab 6A (K144) pAb (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using Rab 6A (K144) #BS3920. “↑”（supernatant); “↓(deposition）

Alternative Name: Ras-related protein Rab-6A; Rab-6; RAB6A; RAB6

Immunogen: Synthetic peptide, corresponding to amino acids 120-170 of Human Rab 6A.

Conjugate: Unconjugated

Modification: Unmodified